江苏大麦纹枯病菌酯酶同工酶的测定 |
| |
引用本文: | 赵桂东,李清铣.江苏大麦纹枯病菌酯酶同工酶的测定[J].菌物学报,1993,12(2). |
| |
作者姓名: | 赵桂东 李清铣 |
| |
作者单位: | 淮阴市农科所,淮阴 223001;江苏农学院植保系,扬州 225001 |
| |
摘 要: | 对立枯丝核菌(Rhizoctonia solani giihn)和禾谷丝核菌(R.E erealis Vande r Hoeven)的16个菌丝融合群或亚群的标准菌株及来自}工苏大麦纹枯病的9个菌丝融合群或亚群共68个菌株进行了聚丙烯酰胺凝胶电泳,测定酯酶同工酶。结果表明:1.立枯丝核菌主酶带数的变幅范围比禾谷丝核菌大;2.无论禾谷丝核菌,还是立枯丝核菌,各菌丝融合群或亚群之间的电泳图谱都有显著差异,但与各自对应的融合群或亚群的标准菌株的图谱则相似;3.44个大麦禾谷丝核菌CAG一1群菌株间的图谱也有差异,但都有一条共同的主酶带(E.);4.据主副酶带数目和位置,将禾谷丝核菌CAG一1群菌株再分为2个类型(1型和II型)和5个亚型(1a、Ib、Ic和Iia、Iib);酶谱类型与菌株的采集品种和致病性无关,但与采集地点似有一定的联系。
|
关 键 词: | 立枯丝核菌 禾谷丝核菌 菌丝融合群 酯酶同工酶电泳图谱 |
DETERMINATION OF ESTERASE ISOENZYMES OF RHIZOCTONIA CEREALIS AND RHIZOCTONIA SOLANI FROM BARLEY IN JIANGSU |
| |
Authors: | ZHAO GUI-DONG Huaiyin Agricultural Research Institute Huaiying LI QING-XIAN Jiangsu Agricultural College Yangzhou |
| |
Institution: | ZHAO GUI-DONG Huaiyin Agricultural Research Institute,Huaiying 223001 LI QING-XIAN Jiangsu Agricultural College,Yangzhou 225001 |
| |
Abstract: | The esterase isoenzyme patterns of 16 standards strains in 16 AGs or sub-AGs and 52 solates from barley in 9 AGs or sub-AGs of Rhizoctonia cerealis and R.solani were ana- lysed with the slab polyacrylamide gel electrophoresis.The results showed:1.Main bands of R.solani were more variable than those of R.cerealis;2.there were marked differences among the patterns of 9 AGs or sub-AGs of R.cerealis and R.solani from barley but similar patterns were shown within AGs or sub-AGs;3.there were some differences among the esterase isoen- zyme patterns of 44 isolates in CAG-1 of R.cerealis from barley but a common main band (E_4) was shaown;4.according to the number and relative position of esterase isoenzyme bands 44 isolates in CAG-1 may be classified into 2 types and 5 sub-types.The esterase patterns had no relation to the pathogenicity or host varieties but they were related to the geographic origins. |
| |
Keywords: | Rhizoctonia cerealis Rhizoctonia solani Anastomosis group Esterase isoenzyme patterns |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |