Purification of glycogen phosphorylase from small quantities of mouse skeletal muscle |
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| Authors: | P E Butler D Fairhurst R J Beynon |
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| Affiliation: | Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, United Kingdom |
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| Abstract: | A new approach to the purification of skeletal muscle glycogen phosphorylase is described. The purification scheme is particularly suited to preparation of the enzyme from small amounts of tissue. A combination of dye-ligand chromatography and hydrophobic chromatography yields homogenous enzyme with good recoveries. The purification is rapid and may be completed in a working day. |
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| Keywords: | glycogen phosphorylase dye-ligand chromatography hydrophobic chromatography protein purification |
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