| 真皮成纤维细胞成骨分化的实验研究 |
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| 引用本文： | 李冬梅,杨水友,李继承.真皮成纤维细胞成骨分化的实验研究[J].中国组织化学与细胞化学杂志,2010,19(6):567-571. |
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| 作者姓名： | 李冬梅 杨水友 李继承 |
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| 作者单位： | 浙江大学医学院基础医学实验教学中心,杭州,310058 |
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| 基金项目： | 浙江省教育厅一般项目(Y200804201) |
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| 摘 要： | 目的比较不同诱导剂对真皮成纤维细胞成骨分化的不同影响,探讨成纤维细胞成骨分化机制。方法取新生大鼠皮肤进行组织块培养,真皮成纤维细胞分离培养及鉴定,并分别由地塞米松、1,25(OH)2D3以及地塞米松和1,25(OH)2D3进行成骨分化诱导。分别于诱导后14d行ALP含量测定,21d行茜素红染色,并进行TAZ表达检测。结果真皮成纤维细胞表达波形蛋白,不表达角蛋白;成纤维细胞诱导14d后,1,25(OH)2D3诱导组及地塞米松+1,25(OH)2D3诱导组ALP含量与对照组有显著差异;成骨诱导21d,地塞米松诱导组仅见少量散在红色钙结节形成,1,25(OH)2D3诱导组钙结节数量增高,地塞米松+1,25(OH)2D3诱导组钙结节数量明显增高,直径变大;1,25(OH)2D3诱导组及地塞米松+1,25(OH)2D3诱导组,经TAZ免疫荧光染色,可见部分细胞核表达TAZ。结论地塞米松可促进1,25(OH)2D3诱导真皮成纤维细胞成骨分化。
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| 关 键 词： | 真皮成纤维细胞 成骨分化 |
EXPERIMENTAL STUDY ON THE DIFFERENTATION OF DERMAL FIBROBLASTS INTO OSTEOBLAST |
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| Li Dongmei,Yang Shuiyou,Li Jicheng.EXPERIMENTAL STUDY ON THE DIFFERENTATION OF DERMAL FIBROBLASTS INTO OSTEOBLAST[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(6):567-571. |
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| Authors: | Li Dongmei Yang Shuiyou Li Jicheng |
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| Institution: | Li Dongmei,Yang Shuiyou,Li Jicheng(Basic Medical Experimental Teaching Center,Medical College of Zhejiang University,Hangzhou 310058,China) |
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| Abstract: | Objective We compared the effects of different revulsions on the differentation from dermal fibroblasts into osteoblasts in order to explore its mechanism.Methods Neonatal rat skin was cultured by tissue pieces.Dermal fibroblasts were isolated,identified,and then induced by dexamethasone,1,25(OH) 2D3,and dexamethasone +1,25(OH) 2D3 for osteogenic differentiation respectively.After 14 days of induction,alkaline phosphatase activity was measured.After 21 days,alizarin red staining and TAZ immunohistochemical ... |
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| Keywords: | Dermal fibroblast Osteoblast differentiation |
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