浙江富阳和海南文昌人工种植长春花生物碱积累组织特异性对比

药用植物长春花的人工栽培分布区主要在我国海南省,目前已引种扩大到浙江、四川等地。为了研究不同栽培区长春花生物碱积累的区域性差异和组织特异性分布特点,本文以浙江富阳和海南文昌两地所产的同苗龄长春花为原料,对其不同部位的长春碱、文朵灵和长春质碱的含量进行对比,分析3种生物碱在不同部位中积累的相关性。研究结果表明,海南文昌所产长春花植株的叶片中3种生物碱含量均高于浙江富阳。但分枝中长春碱含量特点是浙江富阳要显著高于海南文昌;不同部位生物碱之间相关性分析表明,叶片和主茎中文朵灵和长春质碱含量呈显著正相关,而分枝中长春碱与其前体长春质碱成极显著负相关。本文为长春花人工栽培区分布评估提供了理论指导。

Organ-specific Distribution of Alkaloid Accumulations in Catharanthus roseus Artificially Cultivated in Fuyang,Zhejiang Province and Wenchang,Hainan Province

The medicinal plant Catharanthus roseus has been widely cultivated in Hainan province,China in an artificial way and now it was increasingly introduced to other regions,including Zhejiang province and Sichuan province.To reveal the organ-and region-specific properties of alkaloids accumulation in C.roseus seedlings,materials were collected from Zhejiang Fuyang and Hainan Wenchang,and then extracted for alkaloid analysis.The contents of vinblastine,vindoline and catharanthine were respectively measured in leaves,branching twigs,stems and their different combinations.The results showed that these three kinds of alkaloids accumulated in leaves were all higher in seedlings from Hainan than from Zhejiang.However,vinblastine in twigs was significantly higher in Zhejiang seedlings than in Hainan controls.The correlation analysis among vinblastine,vindoline and catharanthine in leaves,stems and twigs indicated that vindoline was positively correlated with catharanthine in leaves and twigs.Vinblastine,however,is significantly related to catharanthine in stems in a negative way.The present paper will provide useful information for region selection when considering artificial cultivation of C.roseus.