The p39 promoter of minute virus of mice directs high levels of bovine growth hormone gene expression in the bovine papilloma virus shuttle vector

The promoter of the capsid-coding genes of the autonomous parvovirus minute virus of mice (MVM) is shown to drive high levels of expression of the heterologous bovine growth hormone (bGH) gene in a bovine papilloma virus (BPV)-based shuttle vector. The expression of bGH directed by the MVM p39 promoter was, on average, higher than that obtained from the widely used metallothionein promoter. These results indicate that the MVM-p39/BPV shuttle vector will be generally useful for the high-level expression of heterologous genes.