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High presence of extended-spectrum beta-lactamases and resistance to quinolones in clinical isolates of Escherichia coli |
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| Authors: | Sorlózano Antonio Gutiérrez José de Dios Luna Juan Oteo Jesús Liébana José Soto María José Piédrola Gonzalo |
| Affiliation: | aDepartments of Microbiology, School of Medicine, San Cecilio University Hospital, University of Granada, Granada bBiostatistics, School of Medicine, San Cecilio University Hospital, University of Granada, Granada cNational Microbiology Centre, Carlos III Institute of Health, Madrid, Spain |
| Abstract: | A study was conducted to detect the presence of extended-spectrum β-lactamases (ESBLs) in 706 isolates of Escherichia coli, largely from outpatients (75.2%). The Clinical and Laboratory Standards Institute (formerly NCCLS)-recommended disk diffusion procedure was used to detect ESBL presence; the VITEK 2 system (bioMérieux, Marcy L’Etoile, France) was used to determine the susceptibility to antibiotics of clinical interest, and the ESBLs were characterized by biochemical study, determining the isoelectric point, and by molecular study with PCR. Clonal distribution was studied in eight hospital isolates. There were 115 ESBL-producing isolates (16.3%), with a predominance of CTX-M9 type (58.3%). We draw attention to the high resistance to quinolones (>70%) in CTX-M9 and SHV enzyme producing isolates and the lower aminoglycoside activity in the latter. |
| Keywords: | Escherichia coli CTX-M9 Quinolones PFGE |
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