| 实时荧光定量PCR方法快速检测转基因大豆 |
| |
| 引用本文： | 朱德斌,邢晓波.实时荧光定量PCR方法快速检测转基因大豆[J].激光生物学报,2012,21(3):279-282. |
| |
| 作者姓名： | 朱德斌 邢晓波 |
| |
| 作者单位： | 华南师范大学生物光子学研究院激光生命科学研究所暨激光生命科学教育部重点实验室 |
| |
| 基金项目： | 国家自然科学基金项目(No.81071790,30600128,6117707);教育部科学技术研究重点项目(No.211131);中国博士后科学基金(No.201003359);广东省自然科学基金项目(No.7005825);广东省优秀博士学位论文作者资助项目(No.SYBZZM201126);广州市南沙区科技计划项目(No.RG201001003) |
| |
| 摘 要： | 针对转基因大豆中普遍含有的35S启动子进行引物设计,以双链DNA染料SYBR GreenⅠ为荧光标记物,利用实时荧光定量PCR方法对大豆样品进行检测。该法检测转基因大豆的检测低限为0.005 nmol/L的35S启动子,线性范围达3个数量级,可快速区分转基因大豆和非转基因大豆,具有快速、简便、灵敏、安全、高通量、低成本等优点,可推广用于转基因植物产品的快速定量检测。
|
| 关 键 词： | 实时荧光定量PCR 转基因大豆 35S启动子 |
Real-time Fluorescence Quantitative PCR Method for Rapid Detection of Genetically Modified Soybean |
| |
| ZHU Debin,XING Xiaobo.Real-time Fluorescence Quantitative PCR Method for Rapid Detection of Genetically Modified Soybean[J].ACTA Laser Biology Sinica,2012,21(3):279-282. |
| |
| Authors: | ZHU Debin XING Xiaobo |
| |
| Institution: | (MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science,College of Biophotonics, South China Normal University,Guangzhou 510631,Guangdong,China) |
| |
| Abstract: | A real-time fluorescence quantitative PCR method was developed to detect genetically modified(GM) soybean using SYBR Green I,a double-stranded DNA-selective fluorescent dye.Special primers were used to amplify 35S promoter that was often used in GM soybeans.The fluorescence of SYBR GreenⅠ was used to monitor the quantity of PCR product.The results show that the detection limit for 35S promoter is 0.005 nmol/L and the linear range is more than three orders of magnitude.The GM soybean and the non-GM soybean can be clearly discriminated.Thus,the method may become a convenient tool for daily GM food detection due to its rapidness,simplicity,sensitivity,safety,high throughput and low cost. |
| |
| Keywords: | real-time fluorescence quantitative PCR genetically modified soybean 35S promoter |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
