Impact of lactate in the perfusate on function and metabolic parameters of isolated working rat heart |
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Authors: | Arzu Onay-Besikci |
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Institution: | (1) Department of Pharmacology, Faculty of Pharmacy, Ankara University, Tandogan 06100, Ankara, Turkey |
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Abstract: | The goal of this study was to investigate the effect of 1 mM exogenous lactate on cardiac function, and some metabolic parameters,
such as glycolysis, glucose oxidation, lactate oxidation, and fatty acid oxidation, in isolated working rat hearts. Hearts
from male Sprague-Dawley rats were isolated and perfused with 5 mM glucose, 1.2 mM palmitate, and 100 μU/ml insulin with or
without 1 mM lactate. The rates of glycolysis, glucose, lactate, and fatty acid oxidation were determined by supplementing
the buffer with radiolabeled substrates. Cardiac function was similar between lactate+ and lactate− hearts. Glycolysis was
not affected by 1 mM lactate. The addition of lactate did not alter glucose oxidation rates. Interestingly, palmitate oxidation
rates almost doubled when 1 mM lactate was present in the perfusate. This study suggests that subst rate supply to the heart
is crucially important when evaluating the data from metabolic studies. |
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Keywords: | cardiac metabolism fatty acid oxidation lactate oxidation |
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