A comparison between inosine- and guanosine-containing anticodons in ribosome-free codon-anticodon binding |
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Authors: | F M Unger S Takemura |
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Institution: | Sandoz Forschungsinstitut GmbH, A-1235 Wien, Austria;Institute of Molecular Biology, Faculty of Science Nagoya University, Nagoya, Japan |
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Abstract: | Binding of the ribooligomers, AUC, AUU, AUA, AUCA, AUUA, and AUAA to the isoleucine-accepting tRNAs, tRNA (anticodon, -IAU-) and tRNA (anticodon, -GAU-) was measured by equilibrium dialysis. With the aid of Scatchard plots, AUCA was shown to bind to one site per tRNA molecule, presumably the anticodon. AUA and AUAA did not measurably attach to either anticodon in the ribosome-free system. All other oligomers were bound to tRNA about 5 to 13 times stronger than to tRNA. |
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