<Emphasis Type="Italic">Halobacillus hunanensis</Emphasis> sp. nov., a moderately halophilic bacterium isolated from a subterranean brine |
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Authors: | Qing-Zhong Peng Qing-Jing Peng Yu-Qin Zhang Zhu-Xiang Liu Yong-Xia Wang Wen-Jun Li Xiao-Long Cui Yi-Guang Chen |
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Institution: | (1) College of Biology and Environmental Sciences, Jishou University, Jishou, 416000, People’s Republic of China;(2) The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China;(3) Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, People’s Republic of China;(4) Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China; |
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Abstract: | A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM
071077T, was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Cells were motile by
means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071077T was able to grow with 2–25% (w/v) total salts (optimum, 5–10%), at pH 6.0–10.0 (optimum, pH 7.5) and 10–40°C (optimum, 25–30°C).
meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7, and the major cellular
fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C15:0. The genomic DNA G+C content was 41.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that
strain JSM 071077T should be assigned to the genus Halobacillus, being related most closely to the type strain of Halobacillus
naozhouensis (98.8% sequence similarity), and the two strains formed a distinct subline in the neighbor-joining, minimum-evolution and
maximum-parsimony phylogenetic trees. The sequence similarities between the novel isolate and the type strains of other recognized
Halobacillus species ranged from 97.6% (with Halobacillus
alkaliphilus) to 95.2% (with Halobacillus kuroshimensis). The results of the phylogenetic analyses, combined with DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic
information, support that strain JSM 071077T represents a new species of the genus Halobacillus, for which the name Halobacillus hunanensis sp. nov. is proposed. The type strain is JSM 071077T (=DSM 21184T = KCTC 13235T). |
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