廊坊杨3号快繁和转基因的再生系统

对杂交杨树新品种廊坊杨3号（Populus langfangensis 3，(P. deltoides (“Shan Hai Guan”)×((P. simonii × pyramidalys)₁₂×Ulmus pumila) 的离体叶片和茎段在附加BA、NAA、IBA和2,4-D的MS培养基上的直接和间接的器官分化、愈伤组织形成和植株再生进行了研究。叶柄和叶片最容易在叶脉处直接诱导生芽。从叶片直接诱导生芽的激素条件为1～2 mg·L^-1 BA和0.5 mg·L^-1 IBA，最高生芽率可达90%。2,4-D促进愈伤组织的形成。由愈伤组织诱导生芽的激素条件为0.3～0.5 mg·L^-1 BA 和 0.02 mg·L^-1 IBA或NAA，生芽率达76%。较好的生根条件为0.1 mg·L^-1 BA和0.2～0.5 mg·L^-1 IBA，生根率可达67％。以上再生条件为廊坊杨3号的转基因育种和无性快繁技术提供了可能。

In-vitro regeneration of Populus langfangensis 3 for transformation and micropropagation

The direct and indirect induction of organ differentiation, callus formation and plantlet regeneration from the leaf and stem explants of the hybrid Populus langfangensis 3 (P. deltoides ("Shan Hai Guan")×((P.simonii × pyramidalys)12×Ulmus pumila) were investigated in-vitro on Murashige and Skoog (MS) medium supplemented with various concentrations of benzylaminopurine (BA), indole 3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D).The petiole and leaf explant was easier for direct shoot regeneration along the veins at cutting sites. Direct shooting efficiency was related to both BA concentration and BA/IBA ratio. Efficient and rapid shooting from the leaf and stem explants was achieved on MS medium containing 1~2 mg·L-1 BA and 0.5 mg·L-1 IBA. Maximum shooting frequency reached 90%. 2,4-D promoted the callus induction of P. langfangensis 3. The shooting based on callus was obtained on MS medium supplemented with 0.3~0.5 mg·L-1 BA and 0.02 mg·L-1 IBA or NAA and the corresponding shooting frequency was 76%. Rooting based on callus succeeded on 0.1 mg·L-1 BA and 0.2~0.5 mg·L-1 IBA, yielding rooting frequency 67%.These regeneration conditions could further be used for micropropagation and transformation of P.langfangensis 3.