Antigen of "serum sickness" type of heterophile antibodies in human sera: indentification as gangliosides with N-glycolylneuraminic acid. |
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| Authors: | H Higashi M Naiki S Matuo K Okouchi |
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| Institution: | Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060 Japan;Department of Laboratory Services, The Hospital of Kyushu University, School of Medicine, Fukuoka 812, Japan |
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| Abstract: | Antigen of “serum-sickness” type of heterophile antibodies in pathologic human sera was purified from equine and bovine erythrocyte stroma. The chemical nature of this antigen was glycosphingolipids with N-glycolylneuraminic acid. The antigen of equine erythrocytes was identified as hematoside with N-glycolylneuraminic acid, GlNeu(α, 2–3)Gal(β, 1–4)Glc(β,1-1) ceramide and the antigen of bovine erythrocytes was N-glycolylneuraminyl-paragloboside, GlNeu (α,2–3)Gal(β,1–4)GlcNAc(β,1–3)Gal(β,1–4)Glc(β,1-1) ceramide. The results indicate that “serum-sickness” antibodies react with a common disaccharide moiety of non-reducing end of the both glycosphingolipids. |
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| Keywords: | GSL glycosphingolipid Gal D-galactose Glc D-glucose GalNAc N-acetyl-D-galactosamine GlcNAc N-acetyl-D-glucosamine AcNeu N-acetylneuraminic acid GlNeu N-glycolylneuraminic acid Cer (ceramide) N-acylsphingosine PBS 0 01 M phosphate buffer (pH 7 0) containing 0 15 M NaCl |
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