Heat-inducible C3HC4 type RING zinc finger protein gene from <Emphasis Type="Italic">Capsicum annuum</Emphasis> enhances growth of transgenic tobacco |
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Authors: | Naheed Zeba Mohammad Isbat Nak-Jung Kwon Mi Ok Lee Seong Ryong Kim Choo Bong Hong |
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Institution: | (1) School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, South Korea;(2) Department of Life Sciences, Sogang University, Seoul, 121-742, South Korea;(3) Present address: Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA |
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Abstract: | Capsicum annuum RING Zinc Finger Protein 1 (CaRZFP1) gene is a novel C3HC4-type RING zinc finger protein gene which was previously isolated from a cDNA library for hot pepper
plants treated of heat-shock. The CaRZFP1 was inducible to diverse environmental stresses in hot pepper plants. We introduced the CaRZFP1 into the Wisconsin 38 cultivar of tobacco (Nicotiana tabacum) by Agrobacterium mediated transformation under the control of the CaMV 35S promoter. Expression of the transgene in the transformed tobacco
plants was demonstrated by RNA blot analyses. There appeared no adverse effect of over-expression of the transgene on overall
growth and development of transformants. The genetic analysis of tested T1 lines showed that the transgene segregated in a Mendelian fashion. Transgenic tobacco lines that expressed the CaRZFP1 gene were compared with several different empty vector lines and they exhibited enhanced growth; they have larger primary
root, more lateral root, larger hypocotyls and bigger leaf size, resulting in heavier fresh weight. Enhanced growth of transgenic
lines accompanied with longer vegetative growth that resulted in bigger plants with higher number of leaves. Microarray analysis
revealed the up-regulation of some growth related genes in the transgenic plants which were verified by specific oligomer
RNA blot analyses. These results indicate that CaRZFP1 activates and up-regulates some growth related proteins and thereby effectively promoting plant growth.
N. Zeba and M. Isbat contributed equally to the work. |
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Keywords: | Capsicum Enhanced growth Heat inducible RING zinc finger protein Transgenic tobacco plants |
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