Introduction of a Micrococcus plasmid in Escherichia coli |
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Authors: | V. Verma G. N. Qazi R. Parshad C. L. Chopra |
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Affiliation: | Genetic Engineering Unit, Regional Research Laboratory, Jammu-Tawi, India. |
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Abstract: | A 6-MDa plasmid (pMQV10), carrying cholesterol hydroxylase and streptomycin-resistance genes, from a gram-positive strain of Micrococcus sps., (RJ6) has been successfully transformed in gram-negative Escherichia coli K12 C600. pMQV10 is maintained stably and expresses its drug resistance in the new host. |
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