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Markers for selection of the rice Xa21 disease resistance gene
Authors:C E Williams  B Wang  T E Holsten  J Scambray  F de Assis Goes da Silva  P C Ronald
Institution:(1) USDA-ARS Crop Production and Pest Control Research Unit and Department of Entomology, Purdue University, 47907 West Lafayette, IN, USA;(2) Department of Plant Pathology, 345 Hutchison Hall, U.C. Davis, 95616 Davis, CA, USA;(3) Present address: Department of Molecular Microbiology, School of Medicine, Washington University, 63110 St. Louis, MO, USA
Abstract:Six molecular markers were mapped to a 7.4-cM region of rice chromosome 11 containing the Xa21 gene, which confers resistance to the pathogen Xanthomonas oryzae pv oryzae. Three markers, RG103, 248 and 818, co-segregated with Xa21 in a population of 1141 plants. Multiple copies of all marker loci were present within the region that was introgressed from Oryza longistaminata into O. sativa. The marker loci were cloned and primers were designed that defined sequence-tagged sites. Physical mapping of the three tightly linked central markers revealed that RG103, the marker that hybridizes to the Xa21 gene, resides on a separate DNA fragment from the other two markers.Disclaimer: Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.
Keywords:Rice  Xa2  Disease resistance  Linkage map  Physical map
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