Differential responses of wood-rot fungi cellulases towards polyclonal antibodies against Trichoderma viride cellobiohydrolase I |
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Authors: | Shoko Uemura Mitsuro Ishihara Jody Jellison |
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Institution: | (1) Forestry and Forest Products Research Institute, Tsukuba Norin Kenkyu Danchi-nai, P. O. Box 16, 305 Ibaraki, Japan;(2) Department of Plant Biology and Pathology, University of Maine, 04469 Orono, ME, USA |
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Abstract: | Cellobiohydrolase (CBH) I, a main component of Trichoderma extracellular protein, was purified to an electrophoretically homogeneous state from a commercial cellulase preparation (Meicelase from T. viride) by column chromatography on anion and cation exchangers. The difference in the cross-reactivity of cellulolytic enzyme systems of brown-rot and white-rot fungi with the polyclonal antibodies to the CBH I was studied by enzyme-linked immunosorbent assay (ELISA). The antibodies were observed to react quantitatively and with great sensitivity with the antigen (CBH I), and at the same time to cross-react to some extent with T. viride cellulase components other than the CBH I. Nevertheless, the intensity of cross-reactivity of wood-rot fungi cellulases with the antibodies was parallel to the activity of exo-1,4-ß-glucanase. The cellulase system from brown-rot fungi, believed to lack exo-1,4-ß-glucanases, gave a negative response towards the antibodies. These results suggested the presence of some homologous sequences and structures with the T. viride CBH I in the enzymes of white-rot fungi and their absence in those of brown-rot fungi.
Correspondence to: M. Ishihara |
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