Flow cytometry of fluorescent proteins |
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Authors: | Telford William G Hawley Teresa Subach Fedor Verkhusha Vladislav Hawley Robert G |
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Affiliation: | Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. |
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Abstract: | ![]() Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use. |
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