Human oral epithelial cell culture II. Keratin expression in fetal and adult gingival cells |
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Authors: | D Oda B A Dale G Bourekis |
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Institution: | (1) Department of Oral Biology, University of Washington, 98195 Seattle, Washington;(2) Department of Periodontology, University of Washington, 98195 Seattle, Washington |
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Abstract: | Summary Epithelial cells from human fetal and adult gingiva were cultured in keratinocyte growth medium (KGM), a serum-free medium.
The expression of keratin proteins in these cells was evaluated using immunohistochemistry and SDS-PAGE-immunoblot analysis
and compared with expression in the tissue. Keratins 5, 6, 14, 16, and 19 were identified in cells cultured from both fetal
and adult tissues. K19 was localized in basal cells of fetal oral tissue but was not seen in adult gingiva (except for scattered
Merkel cells). K1 and K10 were expressed in tissue, but not in cultured cells. The keratin profiles of cultured epithelial
cells from several adult donors were similar and were identical in cultures from primary through Passage 5. K13, a differentiation-specific
keratin, was expressed in all suprabasal cells of fetal oral epithelium, but shows only spotty expression in adult gingival
tissue. K13 was expressed in cultures of fetal cells, but very weakly or not at all in cultures of adult cells. K13 expression
was greater in cultures grown with physiologic calcium concentrations (1.2 mM) than in those grown at 0.15 mM or less. Our findings are consistent with basal-like characters of these cells in 0.15 mM calcium growth conditions. Differentiation of fetal oral cells in culture to the suprabasal basal cell stage in 1.2 mM Ca2+ is shown by the expressionof K13.
This work was supported by Biomedical Research grant RR05346, National Institutes of Health grant DE04660, University of Washington
Graduate Fund and Hack Foundation Fund, Department of Periodontology, University of Washington. |
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Keywords: | K19 K13 oral fetal epithelial culture oral mucosa oral epithelial differentiation |
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