Radioimmunoassay of carbonic anhydrase I and II in rats. Application to the central nervous system during ontogeny] |
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| Authors: | N Limozin D Filippi C Dalmasso G Laurent |
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| Institution: | Equipe de Recherche du C.N.R.S. 49 associée à l''Université d''Aix-Marseille II. Laboratoire de Chimie biologique, Faculté de Médecine, 27, Bd Jean Moulin, 13385 Marseille Cedex 4, France |
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| Abstract: | A specific radioimmunoassay method for rat erythrocyte carbonic anhydrases I and II was developed using a double antibody system. Its sensitivity was in the nanogram range for each of the two isozymes. The method has been applied to the assay of cerebral carbonic anhydrase. Only CAII has been found in brain extracts of perfused rats. Accordingly, the assay of CAI in cerebral tissue can be used to quantify erythrocyte contamination on condition that the ratio CAII/CAI in blood had been worked out. The developmental change in the soluble and the Triton X-100 solubilized brain CAII from birth to adult is reported. |
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| Keywords: | Dosage radioimmunologique Anhydrase carbonique Rat Erythrocytes Système nerveux central CA A qui toute correspondance doit être adressée |
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