Genetic linkage maps of two interspecific grape crosses (Vitis spp.) used to localize quantitative trait loci for downy mildew resistance |
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Authors: | Flavia M Moreira Alberto Madini Rosanna Marino Luca Zulini Marco Stefanini Riccardo Velasco Pal Kozma M Stella Grando |
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Institution: | (1) Istituto Agrario San Michele all’Adige (IASMA) Research and Innovation Centre, Fondazione Edmund Mach, via Edmund Mach 1, 38010 San Michele all’Adige, Italy;(2) Research Institute of Viticulture and Enology, P?zm?ny P?ter u. 4, 7634 P?cs, Hungary;(3) Present address: Resnova S.r.l., via Cadore 14, 00045 Genzano di Roma, Italy;(4) Present address: Instituto di Zootecnia, Universit? Cattolica del Sacro Cuore, Via Emilia Parmese 84, 29122 Piacenza, Italy |
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Abstract: | Two populations (Pop) segregating quantitatively for resistance to downy mildew (DM), caused by Plasmopara viticola, were used to construct genetic maps and to carry out quantitative trait locus (QTL) analysis. Pop1 comprised of 174 F1 individuals from a cross of ‘Moscato Bianco’, a susceptible Vitis vinifera cultivar, and a resistant individual of Vitis riparia. Pop2 consisted of 94 progeny from a cross of two interspecific hybrids, ‘VRH3082 1-42’ and ‘SK77 5/3’, with resistance traits
inherited from Vitis rotundifolia and Vitis amurensis, respectively. Resistance of progeny was measured in field and greenhouse conditions by visual evaluation of disease symptoms
on leaves. Linkage maps of 1037.2 and 651 cM were built essentially with simple sequence repeat markers and were enriched
with gene-derived single-strand conformational polymorphism and single-nucleotide polymorphism markers. Simple interval mapping
and Kruskall–Wallis analysis detected a stable QTL involved in field resistance to DM on linkage group (LG) 7 of the Pop1
integrated map co-localized with a putative Caffeoyl-CoA O-methyltransferase-derived marker. Additional QTLs were detected on LGs 8, 12 and 17. We were able to identify genetic factors
correlated with resistance to P. viticola with lower statistical significance on LGs 1, 6 and 7 of the Pop2 map. Finally, no common QTLs were found between the two
crosses analyzed. A search of the grapevine genome sequence revealed either homologues to non-host-, host- or defense-signalling
genes within the QTL intervals. These positional candidate genes may provide new information about chromosomal regions hosting
phenotypic loci. |
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