Defining the orientation of the tandem fusions that occurred during the evolution of Indian muntjac chromosomes by BAC mapping |
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Authors: | J X Chi L Huang W Nie J Wang B Su F Yang |
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Institution: | (1) Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, 650223, People's Republic of China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, People's Republic of China;(3) Centre for Veterinary Science, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK;(4) Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK |
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Abstract: | The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n=6 in the female and 7 in the male, the karyotypic evolution of which through extensive tandem fusions
and several centric fusions has been well-documented by recent molecular cytogenetic studies. In an attempt to define the
fusion orientations of conserved chromosomal segments and the molecular mechanisms underlying the tandem fusions, we have
constructed a highly redundant (more than six times of whole genome coverage) bacterial artificial chromosome (BAC) library
of Indian muntjac. The BAC library contains 124,800 clones with no chromosome bias and has an average insert DNA size of 120 kb.
A total of 223 clones have been mapped by fluorescent in situ hybridization onto the chromosomes of both Indian muntjac and
Chinese muntjac and a high-resolution comparative map has been established. Our mapping results demonstrate that all tandem
fusions that occurred during the evolution of Indian muntjac karyotype from the acrocentric 2n=70 hypothetical ancestral karyotype
are centromere–telomere (head–tail) fusions. |
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