转录因子结合位点生物信息学研究进展 |
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引用本文: | 侯琳,钱敏平,朱云平,邓明华.转录因子结合位点生物信息学研究进展[J].遗传,2009,31(4):365-373. |
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作者姓名: | 侯琳 钱敏平 朱云平 邓明华 |
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作者单位: | 1. 北京大学数学科学学院, 北京 100871;2. 军事医学科学院放射与辐射医学研究所, 北京蛋白质组研究中心, 蛋白质组学国家重点实验室, 北京 100850;3. 北京大学理论生物学中心, 北京 100871 |
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基金项目: | 国家自然科学基金,国家重点基础研究发展规划(973计划),国家高技术研究发展计划(863计划) |
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摘 要: | By using genome in situ hybridization (GISH) on root somatic chromosomes of allotetraploid derived from the cross Gossypium arboreum × G. bickii with genomic DNA (gDNA) of G. bickii as a probe, two sets of chromosomes, consisting of 26 chromosomes each, were easily distinguished from each other by their distinctive hybridization signals. GISH analysis directly proved that the hybrid G.arboreum×G. bickii is an allotetraploid amphiploid. The karyotype formula of the species was 2n = 4x = 52 = 46m (4sat) + 6sm (4sat). We identified four pairs of satellites with two pairs in each sub-genome. FISH analysis using 45S rDNA as a probe showed that the cross G. arboreum×G. bickii contained 14 NORs. At least five pairs of chromosomes in the G sub-genome showed double hybridization (red and blue) in their long arms, which indicates that chromatin introgression from the A sub-genome had occurred.
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关 键 词: | 系统发育足迹分析法 ChIP-chip 位置权重矩阵 生物信息学 转录因子结合位点 |
收稿时间: | 2008-08-06 |
修稿时间: | 2008-11-05 |
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