Rapid detection of quinolone-resistant Salmonella by real time SNP genotyping |
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| Authors: | Esaki Hidetake Noda Ken Otsuki Noriyuki Kojima Akemi Asai Tetsuo Tamura Yutaka Takahashi Toshio |
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| Affiliation: | National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan. |
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| Abstract: | ![]()
A total of 63 isolates were screened for the gyrA mutation (87Asp-Tyr) in Salmonella enterica serovars using real time PCR. All of the isolates were successfully identified as resistant or susceptible, consistent with the MIC result of the agar dilution method and gyrA sequencing. |
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