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Impact of chromate and dichromate on lysozyme stability: A spectroscopic and molecular docking investigation |
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| Authors: | Suchismita Subadini Ram Sankar Panigrahy Nishesh Kumar Gupta Krishnendu Bera Harekrushna Sahoo |
| Affiliation: | 1. Biophysical and Protein Chemistry Laboratory, Department of Chemistry, NIT Rourkela, Rourkela, India;2. Department of Science, Roma Tre University, Rome, Italy;3. Korea Institute of Civil Engineering and Building Technology, University of Science and Technology, Goyang, Republic of Korea;4. CEITEC MU, Masaryk University, Brno, Czech Republic National Center for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic |
| Abstract: | A comparative study of interaction between chicken egg white lysozyme (Lyz) with two hexavalent chromate ions; chromate and dichromate; which are prevalently known for their toxicity, was investigated using different spectroscopic techniques along with a molecular docking study. Both steady-state and time-resolved studies revealed that the addition of chromate/dichromate is responsible for strong quenching of intrinsic fluorescence in Lyz and the quenching is caused by both static and dynamic quenching mechanisms. Different binding and thermodynamic parameters were also calculated at different temperatures from the intrinsic fluorescence of Lyz. The conformational change in Lyz and thermodynamic parameters obtained during the course of interaction with chromate/dichromate were well-supported by the molecular docking results. |
| Keywords: | anisotropy chromate dichromate fluorescence quenching Lyz |