Anterograde Axonal Transport of Peptidylglycine α-Amidating Monooxygenase in Rat Sciatic Nerves |
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Authors: | Kaeko Tozawa Eiji Arakawa Toshiyuki Chikuma Yoshihiro Oh-hashi Ryuichi Yajima Katsumichi Takeda Hiroshi Shinozaki† Takeshi Kato† |
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Institution: | Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan. |
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Abstract: | Axonal transport of peptidylglycine alpha-amidating monooxygenase (PAM) activity was studied in rat sciatic nerves from 12 to 120 h after double ligations. The anterograde axonal transport increased and reached a plateau between 48 and 72 h and then decreased. The flow rate was 100 mm/day, and the molecular mass of the active entity was 70 kDa, which was determined by gel filtration. In contrast, there was no evidence for significant retrograde axonal transport. Anterograde axonal transport of immunoreactive cholecystokinin, a carboxy-terminal-amidated putative neuropeptide, was also found. These results suggest that PAM is transported by a rapid axonal flow and may play a role as a processing enzyme during transport or in the terminals of rat sciatic nerves. |
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Keywords: | Peptidylglycine amidating enzyme Peptidylglycine cr-amidating monooxygenase Sciatic nerve Axonal transport Cholecystokinin Ligation |
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