聚皂凝胶对蛋白分子的吸附与释放

以牛血清白蛋白为对象，研究了丙烯酸－丙烯酰胺型９ＰＢｕＡＭＡ）和疏水改性的四乙烯五胺－环（ＨＰＴＥ）聚合物凝胶对蛋白质的及附与释放过程。在吸附过程中，当溶液ＰＨ值在牛血清白蛋白电点（４．７）附近时，凝胶蛋白抽的吸附值最大；而在释放过程中，ＰＨ＝７的释放率远大于其它ＰＨ值时的释放率。实验表明凝胶结构对蛋白质吸附与释放有较大影响。未疏水改性的凝胶与疏水改性的凝胶相比，前 的释放速率约为后者的２倍。结果

STUDY OF ABSORPTION AND RELEASE OF PROTEIN ON THE POLYSOAP GLES

Poly(acrylic acid-acrylamide-terbutylacrylamide) gels and stearyl hydrophobically modified poly(tetraethylenepentamine-epichlorohydrin) gels were prepared. The absorption and release of protein on polysoap gels were investigated using Bovine Serum Albumin as a model molecule. There are two processes during the absorption: diffuse of protein into the gels and conjugate of protein on the gels. The maximum absorption was observed when the pH of solutions was near the isoelectric point of protein. A larger release ratio was observed at pH 7. The results indicated that the diffusion of protein into the gels and interaction of protein on the gels play an important role during the process of absorption or release. The release ratio of non-hydrophobically modified gels is 2 times faster than hydrophobically modified gels.