RNA levels in colchicine-resistant mutants of Chlamydomonas reinhardi |
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Authors: | J R Warr Diana Quinn |
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Institution: | Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands |
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Abstract: | A colchicine-resistant mutant of Chlamydomonas reinhardi (col−10, 12) which appears blocked in the final stages of the cell division cycle is shown to have an RNA: protein ratio over four times that which is observed in wild-type cultures. This does not appear to be simply a consequence of reduced overall growth rate, because a comparable reduction in the overall growth rate of wild type by caffeine inhibition did not produce such a large rise in RNA content. The RNA levels in six other colchicine-resistant mutants, which have various abnormalities in colchicine-binding activity, has also been investigated. Two of them have elevated RNA levels. |
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