Agrobacterium tumefaciens-mediated transformation of Robinia pseudoacacia |
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| Authors: | T Igasaki T Mohri H Ichikawa K Shinohara |
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| Institution: | (1) Molecular and Cell Biology Section, Bio-Resources Technology Division, Forestry and Forest Products Research Institute, Ibaraki 305-8687, Japan Fax: +81-298-73-0507 e-mail: kenjis@ffpri.affrc.go.jp, JP;(2) Department of Biotechnology, National Institute of Agrobiological Resources, Ibaraki 305-8602, Japan, JP |
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| Abstract: | Robinia pseudoacacia (black locust) plants were regenerated after co-cultivation of stem and leaf segments with Agrobacterium tumefaciens strain GV3101 (pMP90) that harbored a binary vector that included genes for β-glucuronidase (GUS) and hygromycin phosphotransferase. Successful transformation was confirmed by the ability of stem and
leaf segments to produce calli in the presence of hygromycin, by histochemical and fluorometric assays of GUS activity in
plant tissues, and by Southern blotting analysis. In this transformation system, about 2 months were required for regeneration
of transgenic plants from stem and leaf segments. The frequency of transformation from stem segments was approximately 24%,
and the morphology of regenerated plants resembled that of the original parental strain.
Received: 2 September 1999 / Revision received: 30 November 1999 / Accepted: 4 December 1999 |
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| Keywords: | Agrobacterium tumefaciens Hygromycin phosphotransferase Regeneration Robinia pseudoacacia Transformation |
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