An unusual root tip formation in hairy root culture of Hyoscyamus muticus |
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Authors: | Mondher Jaziri Jacques Homes Koichiro Shimomura |
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Institution: | (1) Tsukuba Medicinal Plant Research Station, National Institute of Health Sciences, 1 Hachimandai, Tsukuba, 305 Ibaraki, Japan;(2) Present address: Université Libre de Bruxelles, Laboratory of Plant Morphology, Chaussée de Wavre 1850, B-1160 Brussels, Belgium |
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Abstract: | Summary Hairy root cultures of Hyoscyamus muticus were established using Agrobacterium rhizogenes ATCC 15834. In one out of 8 clones established, an unusual root tip formation was observed after transfer of cultures from half-strength Murashige and Skoog (1962) to White's medium (1939). This phenomenon was associated with the production of a fine brownish cell suspension culture. Hairy root development resumed after transfer of the root tips from White to half-strength Murashige and Skoog medium. After plating the isolated brownish cells on hormone-free half-strength Murashige and Skoog or White solid medium, callus proliferation was observed, and then redifferentiation of hairy roots occurred. The polymerase chain reaction analysis of the H. muticus hairy root (clone Z2) revealed that only the tl region of the T-DNA was integrated. The growth and the production of five tropane alkaloids by this clone were examined.Abbreviations PCR
Polymerase Chain Reaction
- MS medium
Murashige and Skoog Medium
- 1/2 MS medium
half-strength MS medium
- WP medium
Woody Plant medium
- RC medium
Root Culture medium
- WH medium
White medium
- HPLC
High Performance Liquid Chromatography
- wt.
weight |
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Keywords: | Hyoscyamus muticus Agrobacterium rhizogenes hairy root adventitious root tip tropane alkaloid |
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