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Beta-endorphin-like peptide SLTCLVKGFY reduces the production of 11-oxycorticosteroids by rat adrenal cortex through nonopioid beta-endorphin receptors |
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| Authors: | Navolotskaya Elena V Kovalitskaya Yulia A Zolotarev Yury A Kudryashova Nina Yu Goncharenko Elena N Kolobov Alexander A Kampe-Nemm Elena A Malkova Natalia V Yurovsky Vladimir V Lipkin Valery M |
| Institution: | a Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Science Avenue 6, Pushchino, Moscow 142290, Russia b Institute of Molecular Genetics, Russian Academy of Science, Kurchatov Square 2, Moscow 123182, Russia c Biology Department, Lomonosov Moscow State University, Moscow 117899, Russia d State Research Center for Institute of Highly Pure Bioprepararions, Ministry of Health of the Russian Federation, St. Petersburg 197110, Russia e Department of Medicine, University of Maryland, Baltimore, MD 21201, USA |
| Abstract: | β-Endorphin-like peptide immunorphin (SLTCLVKGFY), a selective agonist of nonopioid β-endorphin receptor, was labeled with tritium to specific activity of 24 Ci/mmol. It was used for the detection and characterization of nonopioid β-endorphin receptors on rat adrenal cortex membranes (Kd=31.6±0.2 nM, Bmax=37.4±2.2 pmol/mg protein). Immunorphin at concentrations of 10?9 to 10?6 M was found to inhibit the adenylate cyclase activity in adrenal cortex membranes, while intramuscular injection of immunorphin at doses of 10–100 μg/kg was found to reduce the secretion of 11-oxycorticosteroids from the adrenals to the bloodstream. |
| Keywords: | Author Keywords: β-Endorphin Nonopioid β-endorphin receptor Adrenal cortex |
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