| 甲基营养型L-丝氨酸产生菌的选育 |
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| 引用本文： | 张超,栾兴社,张维建,侯书国.甲基营养型L-丝氨酸产生菌的选育[J].生物加工过程,2009,7(3):47-50. |
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| 作者姓名： | 张超 栾兴社 张维建 侯书国 |
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| 作者单位： | 山东建筑大学,市政与环境工程学院,济南,250101 |
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| 摘 要： | 以甲基营养型假单胞菌J-12为出发菌株，经硫酸二乙酯（DES）和亚硝基胍（NTG）诱变处理，D-丝氨酸结构类似物平板和高浓度甘氨酸平板定向筛选，获得1株三-丝氨酸高产菌N-13，其发酵液中L-丝氨酸产量较出发菌株提高97．9％。在含有20g/L甘氨酸和7mL／L甲醇的培养基中，L-丝氨酸积累可达4．81g／L。
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| 关 键 词： | 假单胞菌 化学诱变 育种 发酵 L-丝氨酸 |
Breeding of L-serine producing methylotroph mutant |
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| ZHANG Chao,LUAN Xing-she,ZHANG Wei-jian,HOU Shu-guo.Breeding of L-serine producing methylotroph mutant[J].Chinese Journal of Bioprocess Engineering,2009,7(3):47-50. |
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| Authors: | ZHANG Chao LUAN Xing-she ZHANG Wei-jian HOU Shu-guo |
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| Institution: | (School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, China) |
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| Abstract: | L-serine producing methylotroph mutants were derived from Pseudomonas J-12, by mutagenesis with N.methyl-N-nitro-N-nitrosoguanidine（NTG） and diethyl sulfate（DES）. The mutants were selected on the agar plates containing D-serine and high concentration glycine. An L-serine producing mutant N-13 can accumulate about 4.81 g/L L-serine in a medium containing 20 g/L glycine and 7 mL/L of methanol. |
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| Keywords: | Pseudomonas chemical mutagenesis breeding fermentation L-serine |
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