Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells |
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Authors: | Gao Yuan Pagnon Joanne Feng Helen C Konstantopolous Nicky Jowett Jeremy B M Walder Ken Collier Greg R |
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Institution: | Division of Textile and Fibre Technology, Commonwealth Scientific and Industrial Research Organization, Bayview Ave, Clayton 3168, Australia. yuan.gao@csiro.au |
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Abstract: | SEPS1 (also called selenoprotein S, SelS, Tanis or VIMP) is a selenoprotein, localized predominantly in the ER membrane and also on the cell surface. In this report, we demonstrate that SEPS1 protein is also secreted from hepatoma cells but not from five other types of cells examined. The secretion can be abolished by the ER-Golgi transport inhibitor Brefeldin A and by the protein synthesis inhibitor cycloheximide. Using a sandwich ELISA, SEPS1 was detected in the sera of 65 out of 209 human subjects (31.1%, average=15.7+/-1.1 ng/mL). Fractionation of human serum indicated that SEPS1 was associated with LDL and possibly with VLDL. The function of plasma SEPS1 is unclear but may be related to lipoprotein metabolism. |
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Keywords: | DMEM Dulbecco’s modified Eagle’s medium ER endoplasmic reticulum ELISA enzyme-linked immunosorbent assay FBS fetal bovine serum GFP green fluorescence protein IR insulin receptor pGPx extracellular glutathione peroxidase TrxR thioredoxin reductase HDL high density lipoproteins LDL low density lipoproteins VLDL very low density lipoproteins |
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