Proteolytic activity in enzymatic extracts from Carica papaya L. cv. Maradol harvest by-products |
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| Authors: | Galindo-Estrella Thomás Hernández-Gutiérrez Rodolfo Mateos-Díaz Juan Sandoval-Fabián Georgina Chel-Guerrero Luis Rodríguez-Buenfil Ingrid Gallegos-Tintoré Santiago |
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| Institution: | 1. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C, Unidad Sureste, Calle 30 #151 x 7 y 7A, Col. García Ginerés, 97070 Mérida, Yucatán, Mexico;2. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Av. Juarez #421 Cd. Industrial, A.P. 1226-A, 97288 Mérida, Yucatán, Mexico |
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| Abstract: | Proteolytic activity and the cysteine protease profile were determined for enzymatic extracts (EE) from Carica papaya L. cv. Maradol harvest by-products (stems, unripe fruit, petioles and leaves). The proportion of each by-product type in the sampled plantation was calculated. Polypeptide bands were identified by SDS–PAGE for each EE and molecular weight calculated for the cysteine proteases. Leaf and fruit tissue had the highest protein contents of the by-products. Leaf tissue also produced the highest total EE yield. All the SDS–PAGE gels for the EE’s exhibited an approximately 23 kDa band probably corresponding to papain. The zymography profiles of the EE’s were similar, with bands at approximately >202.8, 76.8, 55.4 and 46.5 kDa. The fruit EE had the highest specific proteolytic activity and the leaf EE the lowest. Fruit and stem by-products are the most promising for proteolytic enzyme extraction. |
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