Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system |
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| Authors: | Jung-Min Yoon Masatoshi Nakajima Kiyoshi Mashiguchi Seung-Hyun Park Masato Otani Tadao Asami |
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| Institution: | 1. Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;2. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Japan |
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| Abstract: | We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins’ interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations. |
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