Biosynthesis of linoleic acid in Tyrophagus mites (Acarina: Acaridae) |
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| Authors: | Takako Aboshi Nobuhiro Shimizu Yuji Nakajima Yoshiyuki Honda Yasumasa Kuwahara Hiroshi Amano Naoki Mori |
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| Affiliation: | 1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan;2. Department of Bioenvironmental Science, Kyoto Gakuen University, Kameoka City, Kyoto 621-8555, Japan;3. Division of Environmental Science & Technology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan;4. Yamaguchi Prefectural Agriculture & Forestry, General Technology Center, OouchiMihori, Yamaguchi, Yamaguchi 753-0214, Japan;1. National Dairy Research Institute, Animal Biotechnology Centre, Karnal 132001, India;2. Department of Biochemistry, Banaras Hindu University, 221005 Varanasi, India;1. Department of Gastroenterology and Metabolism, Hiroshima University Hospital, Hiroshima, Japan;2. Division of Frontier Medical Science, Department of Surgery, Programs for Biomedical Research, Graduate School of Biomedical Science, Hiroshima University, Hiroshima, Japan |
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| Abstract: | We report here that Tyrophagus similis and Tyrophagus putrescentiae (Astigmata: Acaridae) have the ability to biosynthesize linoleic acid [(9Z, 12Z)-9, 12-octadecadienoic acid] via a Δ12-desaturation step, although animals in general and vertebrates in particular appear to lack this ability. When the mites were fed on dried yeast enriched with d31-hexadecanoic acid (16:0), d27-octadecadienoic acid (18:2), produced from d31-hexadecanoic acid through elongation and desaturation reactions, was identified as a major fatty acid component of phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) in the mites. The double bond position of d27-octadecadienoic acid (18:2) of PCs and PEs was determined to be 9 and 12, respectively by dimethyldisulfide (DMDS) derivatization. Furthermore, the GC/MS retention time of methyl 9, 12-octadecadienoate obtained from mite extracts agreed well with those of authentic linoleic acid methyl ester. It is still unclear whether the mites themselves or symbiotic microorganisms are responsible for inserting a double bond into the Δ12 position of octadecanoic acid. However, we present here the unique metabolism of fatty acids in the mites. |
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| Keywords: | Biosynthesis of linoleic acid Δ12-Desaturase Essential fatty acids CDL" },{" #name" :" keyword" ," $" :{" id" :" kwrd0035" }," $$" :[{" #name" :" text" ," _" :" curved desolvation line DMDS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" dimethyldisulfide ESI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" electron spray ionization GC/MS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" gas chromatography mass spectrometry LC/MS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" liquid chromatography mass spectrometry LPC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" lysophosphatidylcholine LPE" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" lyso phospatidylethanolamine PC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" phosphatidylcholine PE" },{" #name" :" keyword" ," $" :{" id" :" kwrd0115" }," $$" :[{" #name" :" text" ," _" :" phosphatidylethanolamine PI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0125" }," $$" :[{" #name" :" text" ," _" :" phosphatidylinositol |
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