Mitochondrial dysfunction contributes to the cytotoxicity of some 3,5-bis(benzylidene)-4-piperidone derivatives in colon HCT-116 cells |
| |
Authors: | Muath Helal Umashankar Das Brian Bandy Azharul Islam Adil J Nazarali Jonathan R Dimmock |
| |
Institution: | 1. Drug Design and Development Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5C9;2. Vaccine and Infectious Disease Organization-International Vaccine Center (VIDO-Intervac), University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E3 |
| |
Abstract: | The objectives of this study are to investigate the possible ways by which the curcumin analogs 2a and 2b exert their antiproliferative properties. The analogs 2a and 2b have submicromolar IC50 values towards human HCT-116 colon cancer cells but are far less toxic to human non-malignant CRL-1790 colon cells. Both compounds affected a number of mitochondrial functions in HCT-116 cells namely increasing the intracellular concentrations of reactive oxygen species, inhibiting oxygen consumption and decreasing the mitochondrial membrane potential. These molecules also produced swelling of isolated rat liver mitochondria, supporting a mitochondrial mechanism of cytotoxicity. Both compounds reacted with glutathione in the presence of glutathione S-transferase π and hence they may be classified as thiol alkylators. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|