Synthesis,crystal structures,DNA binding and cytotoxicity of two novel platinum(II) complexes containing 2-(hydroxymethyl)pyridine and pyridine-2-carboxylate ligands |
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Authors: | Ceyda Icsel Veysel T Yilmaz Aysegul Golcu Engin Ulukaya Orhan Buyukgungor |
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Institution: | 1. Department of Chemistry, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey;2. Department of Chemistry, Faculty of Science and Arts, University of Kahramanmara? Sütçü Imam, Campuse of Avsar, 46100 Kahramanmaras, Turkey;3. Department of Medical Biochemistry, Medical School, Uludag University, 16059 Bursa, Turkey;4. Department of Physics, Faculty of Arts and Sciences, Ondokuz Mayis University, 55139 Samsun, Turkey |
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Abstract: | Two new platinum(II) complexes, trans-Pt(2-mpy)2]·4H2O (1) and PtCl(2-pyc)(2-hmpy)]·H2O (2), where 2-hmpy = 2-(hydroxymethyl)pyridine, 2-mpy = deprotonated 2-hmpy and 2-pyc = pyridine-2-carboxylate, have been synthesized and characterized by elemental analysis, IR, NMR, and X-ray crystallography. The DNA binding affinities of these complexes for Fish Sperm DNA (FS-DNA) were investigated using fluorescence, viscosity, thermal denaturation and gel electrophoresis measurements. Fluorescence analysis indicates that complex 1 binds to DNA by a single intercalative mechanism, while complex 2 exhibits two types of interactions such as intercalation and covalent binding. Gel electrophoresis assay demonstrates ability of the complexes to cleavage the supercoiled pBR322 plasmid DNA. The in vitro cytotoxicities of both complexes were preliminarily evaluated and the cytotoxicity of complex 1 against the human lung cancer cells (H1299) is similar to oxaliplatin, but higher than transplatin and carboplatin. |
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