The use of cellulose xanthate for the immobilisation of biological molecules |
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Authors: | John F Kennedy Ahmad Zamir |
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Institution: | Department of Chemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT Great Britain |
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Abstract: | The mercapto groups of cellulose xanthate can reversibly form disulphide bridges with L-cysteine. This property has been utilised for the immobilisation of a protein and an enzyme. These macromolecules, as polythiol derivatives, formed disulphide linkages with the matrix without serious disturbance of their active sites, became firmly bound to the xanthate, and were not eluted by normal washing conditions. Cellulose xanthate is a cheap, easily prepared matrix which permits a simple coupling reaction. The immobilisation process is selectively reversible. |
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