Competing Factors of Compost Concentration and Proximity to Root Affect the Distribution of Streptomycetes |
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Authors: | Ehud Inbar Stefan J Green Yitzhak Hadar Dror Minz |
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Institution: | (1) Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot, Israel;(2) Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel |
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Abstract: | Streptomycetes are important members of soil microbial communities and are particularly active in the degradation of recalcitrant
macromolecules and have been implicated in biological control of plant disease. Using a streptomycetes-specific polymerase
chain reaction (PCR)-denaturing gradient gel electrophoresis (PCR-DGGE) methodology coupled with band excision and sequence
analysis, we examined the effect of grape marc compost amendment to soil on cucumber plant–associated streptomycetes community
composition. We observed that both compost amendment and proximity to the root surface influenced the streptomycetes community
composition. A strong root selection for a soil-derived Streptomycete, most closely related to Streptomyces thermotolerans, S. iakyrus, and S. thermocarboxydus, was independent of compost amendment rate. However, while the impact of compost amendment was mitigated with increasing
proximity to the root, high levels of compost amendment resulted in the detection of compost-derived species on the root surface.
Conversely, in rhizosphere and non-rhizosphere soils, the community composition of streptomycetes was affected strongly even
by modest compost amendment. The application of a streptomycetes-specific PCR primer set combined with DGGE analysis provided
a rapid means of examining the distribution and ecology of streptomycetes in soils and plant-associated environments. |
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