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Biotransformation of Phenylacetonitrile to 2-Hydroxyphenylacetic Acid by Marine Fungi |
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| Authors: | Julieta Rangel de Oliveira Carolina Megumi Mizuno Mirna Helena Regali Seleghim Darci Consolação Diniz Javaroti Maria Olímpia Oliveira Rezende Maria Diva Landgraf Lara Durães Sette André Luiz Meleiro Porto |
| Institution: | 1. Instituto de Química de S?o Carlos, Universidade de S?o Paulo, Av. Trabalhador S?o-carlense, 400, CEP 13560-970, CP 780, S?o Carlos, SP, Brazil 2. Departamento de Ecologia e Biologia Evolutiva, Universidade Federal de S?o Carlos, Via Washington Luís, Km 235, S?o Carlos, SP, CEP 13565-905, Brazil 3. Divis?o de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, CPQBA, Universidade Estadual de Campinas, Rua Alexandre Casellato, 999, CEP 13140-000, Paulínia, SP, Brazil |
| Abstract: | Marine fungi belonging to the genera Aspergillus, Penicillium, Cladosporium, and Bionectria catalyzed the biotransformation of phenylacetonitrile to 2-hydroxyphenylacetic acid. Eight marine fungi, selected and cultured with phenylacetonitrile in liquid mineral medium, catalyzed it quantitative biotransformation to 2-hydroxyphenylacetic acid. In this study, the nitrile group was firstly hydrolysed, and then, the aromatic ring was hydroxylated, producing 2-hydroxyphenylacetic acid with 51 % yield isolated. In addition, the 4-fluorophenylacetonitrile was exclusively biotransformed to 4-fluorophenylacetic acid by Aspergillus sydowii Ce19 (yield?=?51 %). The enzymatic biotransformation of nitriles is not trivial, and here, we describe an efficient method for production of phenylacetic acids in mild conditions. |
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