A method for separate quantitative determination of 2-keto-3-deoxy-octonate and 3,6-dideoxyhexoses in mixtures. |
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| Authors: | T I Burtseva L I Glebko Y S Ovodov |
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| Affiliation: | Institute of Medical Chemistry, Biomedical Center, University of Uppsala, Box 575, S-751 23 Uppsala, Sweden |
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| Abstract: | A method for analysing 32P in small aqueous samples by measuring the ?erenkov radiation is described. Centering problems with small sample volumes are eliminated by placing the sample in a polystyrene tube in the scintillation vial. The detection efficiency is high, 54.5 ± 0.6% (2 SD) at a sample volume of 25 μl. The reproducibility is good and independent of the sample volume. The detection efficiency of 32P in polyacrylamide gel is shown to be as good as in water. |
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