PVAX-MAGE-1基因疫苗延缓小鼠肝癌细胞H22腹水瘤和实体瘤的生长

为观察重组基因疫苗PVAX-MAGE-1的抑瘤效应,构建黑色素瘤抗原-1(melanoma antigen-1,MAGE-1)真核基因表达载体--PVAX-MAGE-1.以重组质粒免疫C57BL/6小鼠后,ELISA法检测表明,与对照鼠(PVAX-1和生理盐水注射小鼠)比较,免疫小鼠脾淋巴细胞上清液中的细胞因子IL-2和IFN-γ明显升高(P0.05);淋巴细胞-肿瘤细胞混合培养证明,免疫小鼠外周血CD8+T细胞对靶细胞的特异性杀伤作用明显增强(P0.05).体内实验证明,PVAX-MAGE-1免疫C57BL/6小鼠,可显著延缓移植性H22腹水瘤及实体瘤在小鼠体内的生长.实验结果提示,重组基因疫苗PVAX-MAGE-1有明显的延缓肿瘤生长的作用,其抑瘤作用与提高T淋巴细胞IL和IFN表达,增强对肿瘤杀伤作用直接相关.

Growth Inhibition of Mouse Ascites and Solid Hepatoma 22With PVAX-MAGE-1 Gene Vaccine

The melanoma antigen-1(MAGE-1) gene was inserted into a mammalian expression vector PVAX-1 and used to vaccinated C57BL/ 6 mice using saline as the controls. The splenocyte-secreted IL-2 and IFN-γ were significantly higher than those of the controls (P <0.05), as well as the CTL activity of CD8+ T cells (P<0.05). In vivo experiments showed that the immunized C57BL/6 mice could inhibit the growth of the transplanted H22 ascites and solid tumor. Our results indicated that PVAX-MAGE-1 gene vaccine was able to suppress the tumor growth, and might involve the enhanced expression of IL and IFN.