Heterogeneity in the surface properties of B16 melanoma cells from sublines with differing metastatic potential detected via two-polymer aqueous-phase partition |
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| Authors: | James M. Van Alstine Poul Sorensen Timothy J. Webber Russell Greig George Poste Donald E. Brooks |
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| Affiliation: | 1. Department of Pathology University of British Columbia, Vancouver, BC, Canada V6T 1W5;2. Department of Chemistry, University of British Columbia, Vancouver, BC, Canada V6T 1W5;3. Department of Tumour Biology, L109 Smith, Kline and French Laboratories, Philadelphia, PA 19101, USA;4. Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA;1. National Center for Physical Acoustics and Department of Physics and Astronomy, University of Mississippi, University, Mississippi, USA;2. Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA;1. Division of Gastroenterology, Department of Medicine, University of Washington, Seattle, WA, USA;2. Center for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, WA, USA;3. Department of Urology, University of Washington School of Medicine, Seattle, WA, USA;4. Faculty of Physics, M.V. Lomonosov Moscow State University, Moscow, Russia;1. Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA;2. Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA;3. Division of Pediatric Cardiology, Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, USA;1. Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA;2. Department of Urology, University of Michigan, Ann Arbor, MI, USA;3. Department of Pathology, University of Michigan, Ann Arbor, MI, USA;4. Department of Ultrasound, Shanghai Jiaotong University, Shanghai, China;5. Department of Surgery, University of Michigan, Ann Arbor, MI, USA;6. Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, USA;1. Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA;2. Department of Biomedical Engineering, Schools of Engineering and Natural Science, Istanbul Medipol University, Istanbul, Turkey;3. Department of Radiology, University of Michigan, Ann Arbor, MI, USA;4. Division of Pediatric Cardiology, Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, USA;5. Macromolecular Science and Engineering Program, University of Michigan, Ann Arbor, MI, USA |
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| Abstract: | ![]()
When mixed in aqueous solution at low concentrations, the neutral polymers dextran and poly(ethylene glycol) (PEG) rapidly form a two-phase system, consisting of a dextran-enriched lower phase and a PEG-enriched upper phase. Two B16 mouse melanoma cell lines, B16-F1 (low lung colonizing capability) and B16-F10 (high lung colonizing capability) were found to partition differentially into the upper phase in a variety of two-phase systems. Upper-phase partition depends primarily on either hydrophilic (i.e., surface charge density) or hydrophobic (i.e., affinity for the hydrocarbon chain of a PEG-fatty acid ester) cell surface properties, depending on the system used. In single-step partition studies, cells of the B16-F10 subline displayed a greater preference than B16-F1 cells for the upper phase in the hydrophilic system and less preference in systems sensitive to hydrophobic properties. Countercurrent distribution (CCD) experiments, performed with [125I]deoxyuridine DNA-labelled cells, were consistent with single-step partition results. These CCD results demonstrated that B16-F10 cells exhibited greater DNA synthesis than B16-F1 cells and that considerable heterogeneity, in both hydrophobic and hydrophilic surface properties, was present in subpopulations of cells of both sublines. The data also showed considerable enrichment of 125I-specific cell activity in certain sections of the distributions, indicating that differences in cellular DNA synthesis are reflected in the surface properties to which partition is sensitive. |
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