褐飞虱不同致害性种群体内共生菌18S rDNA部分序列比较

分离纯化了褐飞虱3种不同致害性种群体内类酵母共生菌 (yeast-like symbionts, YLS)，并对其18S rDNA基因序列进行了比较。结果表明，褐飞虱3种不同致害性种群体内类酵母共生菌18S rDNA均扩增出600 bp左右的片断。依据获得的18S rDNA特异性序列，结合已知真菌的18S rDNA部分序列，构建了不同宿主的YLS分子系统树。结果显示， 褐飞虱3种不同致害性种群体内的YLS同属子囊菌亚门（Ascomycotina）的核菌纲（Pyrenomycetes），并与此纲中的Hypomyces chrysospermus亲缘关系相对最近。

The comparison of yeast-like symbionts in the brown planthopper,Nilaparvata lugens St(a)l with different virulence based on partial 18S rDNA sequence

The yeast-like symbionts (YLS) were isolated and purified from the fat body of the brown planthopper, Nilaparvata lugens Stal (BPH) with different virulence reared on various rice resistant varieties. The 18S rDNA sequences of YLS from BPH with different virulence were analyzed and compared with the sequences of other fungi explored from GenBank, and a comprehensive phylogenetic tree was constructed. The phylogenetic analyses suggested that the YLS of BPH with different virulence belonged to the subphylum Ascornycotina, the class Pyrenomycetes, and had the closest genetic relationship with Hypomyces chrysospermus.