Species and Organ Diversity in the Effects of Hydrogen Peroxide on Superoxide Dismutase Activity In Vitro |
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作者姓名: | Hong-Yan Cheng Song-Quan Song |
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作者单位: | [1]Xishuangbanna Tropical Botanical Garden, the Chinese Academy of Sciences, Mengla, Yunnan 666303, China [2]Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China |
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基金项目: | Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-SW-117), Hundreds Talent Program of the Chinese Academy of Sciences, Natural Science Foundation of Yunnan, China (2003C0068M), and the Ministry of Sciences and Technology of China (2004DKA30430).Acknowledgments The authors thank Miss Fang Huang for some experimental help and Miss Meihua Tian for purification of jackfruit mitochondria. |
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摘 要: | Superoxlde dlsmutase (SOD) is ubiquitous in aerobic organisms and constitutes the first link In the enzyme scavenging system of reactive oxygen species. In the present study, species and organ diversity of SOD activity In a solution and In an in-gel assay system, as well as the effects of hydrogen peroxide (H202) on SOD activity, were Investigated. In a solution assay system, SOD activity of jackfruIt root, shoot, leaves, axes, and cotyledons, of maize embryos and endosperms, of mung bean leaves and seeds, of sacred lotus axes and cotyledons, and of rice and wheat leaves was Increased by 1-15 mmol/L H2O2. However, SOD activity In rice root and seeds, maize roots and leaves, mung bean roots and shoots, and wheat seeds was decreased by 1-15 mmol/L H2O2. The SOD activity of wheat root and soybean roots, leaves, axes, and cotyledons was Increased by 1-4 mmol/L H2O2, but was decreased by concentrations of H2O2 〉4 mmol/L. The SOD activity of soybean shoots was not affected by 1-15 mmol/L H2O2. The SOD activity In crude mltochondrla of jackfruIt, maize, and upas seeds, as well as In purified mitochondria of jackfruIt, was also Increased by 1-15 mmol/L H2O2. In the In-gel assay system, the SOD In jackfruIt cotyledons was comprised of Mn-SOD, Cu/Zn-SOD, and Fe-SOD, the crude mltochondria of jackfruit seeds and maizes embryo was comprised of Mn-SOD and Cu/ Zn-SOD, and the crude mltochondria of maize seeds was comprised of Mn-SOD only. In the present study, H2O2 markedly Inhibited Cu/Zn-SOD and Fe-SOD activity.
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关 键 词: | 种类多样性 种子 过氧化氢 放射性活度 过氧化物歧化酶 |
收稿时间: | 2005-08-18 |
修稿时间: | 2005-08-182005-11-08 |
Species and Organ Diversity in the Effects of Hydrogen Peroxide on Superoxide Dismutase Activity In Vitro |
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Authors: | Hong-Yan Cheng Song-Quan Song |
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Institution: | (Xishuangbanna Tropical Botanical Garden the Chinese Academy of Sciences;, Mengla, Yunnan 666303, China;Institute of Botany, the Chinese Academy of Sciences;, Beijing 100093, China) |
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Abstract: | Superoxide dismutase (SOD) is ubiquitous in aerobic organisms and constitutes the first link in the enzyme scavenging system of reactive oxygen species. In the present study, species and organ diversity of SOD activity in a solution and in an in-gel assay system, as well as the effects of hydrogen peroxide (H2O2) on SOD activity, were investigated. In a solution assay system, SOD activity of jackfruit root, shoot, leaves, axes, and cotyledons, of maize embryos and endosperms, of mung bean leaves and seeds, of sacred lotus axes and cotyledons, and of rice and wheat leaves was increased by 1-15 mmol/L H2O2. However, SOD activity in rice root and seeds, maize roots and leaves, mung bean roots and shoots, and wheat seeds was decreased by 1-15 mmol/L H2O2. The SOD activity of wheat root and soybean roots, leaves, axes, and cotyledons was increased by 1-4 mmol/L H2O2, but was decreased by concentrations of H2O2 >4 mmol/L. The SOD activity of soybean shoots was not affected by 1-15 mmol/L H2O2. The SOD activity in crude mitochondria of jackfruit,maize, and upas seeds, as well as in purified mitochondria of jackfruit, was also increased by 1-15 mmol/L H2O2. In the in-gel assay system, the SOD in jackfruit cotyledons was comprised of Mn-SOD, Cu/Zn-SOD, and Fe-SOD, the crude mitochondria of jackfruit seeds and maizes embryo was comprised of Mn-SOD and Cu/Zn-SOD, and the crude mitochondria of maize seeds was comprised of Mn-SOD only. In the present study,H2O2 markedly inhibited Cu/Zn-SOD and Fe-SOD activity. |
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Keywords: | hydrogen peroxide inhibitor informative in-gel assay system solution assay system species and organ diversity superoxide dismutase activity |
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