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Characterization of progenies of Triticum aestivum- Psathyrostachys juncea derivatives by using genomic in-situ hybridization
Authors:Ronghua Zhou  Jizeng Jia  Yuchen Dong  T Schwarzacher  T S Miller  S Reader  Sh B Wu and M D Gale
Institution:(1) Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, 100081 Beijing, China;(2) John Innes Centre, NR4 7UH Norwich
Abstract:Using genomicin-situ hybridization (GISH) technique, 7 translocation-addition lines, 6 translocation and translocation-addition lines, 2 ditelosomic addition lines and 1 translocation line were identified fromTriticum aestivum L. -Psathyrostachys juncea (Fisch.) Nevski intergeneric hybrids, of which translocation-addition and translocation and translocation-addition lines were not found in other reports. No substitutions and disornic additions were detected in the, hybrids and breakages occurred in allP. juncea chromosomes studied. Results have shown that the improved GISH technique is a rapid and economical method for use in this field.
Keywords:Triticum aestivum                      Psathymstachys juncea            translocation-addition  translocation and translocation-addition            in-situ hybridization
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