| 重组人血清白蛋白发酵过程表达期的定量研究 |
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| 引用本文： | 黄明志,郭美锦,储炬,杭海峰,庄英萍,张嗣良.重组人血清白蛋白发酵过程表达期的定量研究[J].生物工程学报,2003,19(1):81-86. |
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| 作者姓名： | 黄明志 郭美锦 储炬 杭海峰 庄英萍 张嗣良 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心,生物工程学院,上海,200237 |
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| 基金项目： | 国家自然科学基金 (No:2 99760 13 )，上海市重点学科基金资助~~ |
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| 摘 要： | 结合元素平衡和代谢平衡，建立了重组人血清白蛋白发酵过程表达期的数学模型，按单纯形多变量最优化方法估算了模型的未知参数，并探讨了导致表达期不同阶段产物形成速率存在差异的可能原因。该模型能较好地描述重组人血清白蛋白发酵过程表达期中各宏观反应速率之间的关系，为重组人血清白蛋白的高效表达提供了线索。
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| 关 键 词： | 重组人血清白蛋白(rHSA) 代谢平衡 元素平衡 Pichia pastoris |
| 文章编号： | 1000-3061(2003)01-0081-06 |
| 修稿时间： | 2002年7月18日 |
Quantitative Studies of the Production Phase in rHSA Fermentation |
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| HUANG Ming Zhi,GUO Mei Jin CHU Ju HANG Hai Feng ZHUANG Ying Ping ZHANG Si Liang.Quantitative Studies of the Production Phase in rHSA Fermentation[J].Chinese Journal of Biotechnology,2003,19(1):81-86. |
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| Authors: | HUANG Ming Zhi GUO Mei Jin CHU Ju HANG Hai Feng ZHUANG Ying Ping ZHANG Si Liang |
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| Institution: | State Key Laboratory of Bioreactor Engineering, National Center of Biotechnology, Bioengineering College, East China University of Science and Technology, Shanghai 200237, China. huangmingzhi@263.net.cn |
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| Abstract: | The model equations of the production phase of rHSA fermentation were derived on the base of both elemental balance and metabolic balance, then the unknown parameters of the model were estimated by multivariable optimization The possible reasons of discrepancy of production rate between different period of fermentation were discussed The model could preferably described the relations between different macroscopic reaction rates of the process and keys for the high efficiency expression of HAS were deduced |
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| Keywords: | recombinant human serum albumin (rHSA) metabolic balance elemental balance Pichia pastoris |
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