Purification and characterization of α-glucosidases produced by Saccharomyces in response to three distinct maltose genes |
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Authors: | Shiro Tabata Takeshi Ide Yasuyoshi Umemura Kenzo Torri |
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Institution: | Department of Chemistry, Nara Medical University, Kashihara, Nara 634, Japan |
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Abstract: | α-Glucosidases or maltases (EC 3.2.1.20) were purified to electrophoretic homogeneity from a respective strain of Sacchromyces cerevisiae which carries a single MAL gene, either MALα, MALβ or MALγ, using gluconate-Sepharose affinity chromography and isoelectrofocusing. Of these maltases, two types of maltase were obtained from the MALγ strain, the pI values of which were 5.6 and 5.9. From the MALα and MALβ strain was obtained only one type of maltase with the pI at 5.6 which was identical to one of the maltases from the MALγ strain. These four maltases possessed the same properties, except for pI. They were monomers with molecular weights of between 66 000 and 67 000. With regard to the substrate specificity, they hydrolyzed maltose and sucrose exclusively but not α-methulglucoside nor maltooligosaccharide. They did not differ in immunological properties. |
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Keywords: | α-Glucosidase Maltose gene (Saccharomyces) |
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