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The structure of the carbohydrate units of the carboxyl-terminal peptide of procollagen as elucidated by 500 MHz 1H-NMR spectroscopy |
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| Authors: | Thomas Marti Phyllis A Kosen Kazik Honory Carl Franzblau Karl Schmid Herman Van Halbeek Gerrit J Gerwig Johannes FG Vliegenthart | |
| Institution: | 1. Department of Biochemistry, Boston University School of Medicine, 80 East Concord Street, Boston, MA 02118, U.S.A.;2. Department of Bio-Organic Chemistry, University of Utrecht, Croesestraat 79, 3522 AD Utrecht, The Netherlands | |
| Abstract: | The oligosaccharides of chick embryo type I procollagen were isolated from the carboxyl-terminal propeptide fragment by exhaustive digestion with papain and pronase, and then purified as a mixture of glycopeptides. The structures of the oligosaccharides were established by high-resolution 1H-NMR spectroscopy and found to be a mixture with respect to the non-reducing terminal residues as shown below:  | |
| Keywords: | Carbohydrate sequence Procollagen C-terminal Peptide | |
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