| 三角褐指藻(Phaeodactylum tricornutum)通用转化载体的构建 |
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| 引用本文： | 郑国庭,姜鹏,秦松,严小军.三角褐指藻(Phaeodactylum tricornutum)通用转化载体的构建[J].生物学杂志,2012,29(4):8-11. |
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| 作者姓名： | 郑国庭 姜鹏 秦松 严小军 |
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| 作者单位： | 1. 宁波大学,浙江省海洋生物工程重点实验室,宁波,315211
2. 中国科学院海洋研究所,青岛,266071 |
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| 基金项目： | 973课题，863课题，山东省博士基金 |
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| 摘 要： | 三角褐指藻(Phaeodactylum tricornutum)是开展微藻生物柴油研究的理想材料。克隆了内源fcp基因簇的多个调控序列(启动子、终止子),构建了包括fcpB启动子-bar基因-fcpA终止子、以及fcpA启动子-多克隆位点(MCS)-fcpA终止子两个表达盒的通用转化载体pfcpA-MCS/fcpB-Bar,其特征是以bar基因作为选择标记,MCS区方便插入一至多个目的基因。新载体可用于三角褐指藻的重组蛋白表达、或油脂代谢相关基因的功能验证和代谢调控研究。
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| 关 键 词： | bar基因 三角褐指藻 通用转化载体 |
Construction of a transformation vector for diatom Phaeodactylum tricornutum |
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| ZHENG Guo-ting
,
JIANG Peng
,
QIN Song
,
YAN Xiao-jun.Construction of a transformation vector for diatom Phaeodactylum tricornutum[J].Journal of Biology,2012,29(4):8-11. |
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| Authors: | ZHENG Guo-ting JIANG Peng QIN Song YAN Xiao-jun |
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| Institution: | 1. Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211 ; 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China ) |
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| Abstract: | Phaeodactylum tricornutum is an ideal material for biodiesel research. In this paper, several endogenous elements, i.e. promoters and terminators, from fcp （fucoxanthin, chlorophyll a/c-binding protein） gene clusters were cloned, and a transformation vector pfcpA-MCS/fcpB-bar including two expression cassettes which were fcpB promoter-bar gene-fcpA terminatiom and fcpA promoter-MCS （multiple cloning site）-fcpA terminatiom was constructed and characterized by using bar gene as selection marker. It carried a large MCS easy for insertion of one or more function genes. This vector will be used for lipid metabolic engineering for Phaeodactylum tricornutum. |
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| Keywords: | bar gene Phaeodactylum tricornutum general transformation vector |
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